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This Concept Map, created with IHMC CmapTools, has information related to: LAB 1- INTRODUCTION TO THE MICROSCOPE AND COMPARISON OF SIZES AND SHAPES OF MICROORGANISMS, spiral forms spirochete: thin, flexible spiral, coccus: spherical or oval arrangements staphylococcus: cocci in irregular, grape-like clusters; division in random planes, Focusing with oil immersion microscopy. (1000X) step 1 Before you plug in the microscope, turn the voltage control dial on the right-hand side of the base of the microscope to 1). Now plug in the microscope and turn it on., bacillus (rod): hot dog-shaped size typically 0.5-1.0 µm wide and 1- 4 µm long, common bacterial shapes shape name coccus: spherical or oval, Rotate the white-striped 100X oil immersion objective until it is locked into place. This will give a total magnification of 1000X. step 4 Make sure the iris diaphragm lever in front under the stage is almost wide open, (toward the left side of the stage.), Yeast are eukaryotic unicellular fungi that divide by budding microscopic appearance baker's yeast, common bacterial shapes shape name spiral, common bacterial shapes shape name bacillus (rod): hot dog-shaped, coccus: spherical or oval arrangements diplococcus: cocci in pairs; division in one plane, Bacteria are unicellular prokaryotic microorganisms that divide by binary fission. microscopic appearance common bacterial shapes, bacillus (rod): hot dog-shaped arrangements bacillus: single bacillus, Place the slide in the slide holder and place a rounded drop of immersion oil on the area to be observed. step 3 Rotate the white-striped 100X oil immersion objective until it is locked into place. This will give a total magnification of 1000X., spiral forms vibrio: comma-shaped, coccus: spherical or oval size typically 0.5-1.0 µm in diameter, spiral forms spirillum: thick, rigid spiral, baker's yeast size 3 - 5 µm in diameter, While looking through the eyepieces, turn the fine focus (smaller knob) towards you at a slow steady speed until the specimen comes into focus. step 7 Using the iris diaphragm lever, adjust the light to obtain optimum contrast, Watching the slide and objective lens carefully from the front of the microscope, lower the oil immersion objective into the oil by raising the stage until the lens just touches the slide. Do this by turning the coarse focus (larger knob) away from you until the spring-loaded objective lens just begins to spring upward. step 6 While looking through the eyepieces, turn the fine focus (smaller knob) towards you at a slow steady speed until the specimen comes into focus., Using the iris diaphragm lever, adjust the light to obtain optimum contrast measuring the size of a microorganism Use an ocular micrometer. At 1000 magnification, the distance between any two lines represents approximately 1 µm.
